hCB1
Nomenclature
C57BL/6Smoc-Cnr1em1(hCB1)Smoc
Cat. NO.
NM-HU-225027
Strain State
Repository Live
Gene Summary
Gene Symbol
Cnr1
Model Description
The endogenous mouse Cnr1 gene was replaced by human CB1 gene.
Validation Data
Fig1. Detection of CB1 expression in cerebellum by RT-PCR.
Wild type: only one band at 373 bp with primers F1/R1(mCb1);
Homozygous: only one band at 209 bp with primers F2/R2(hCB1);
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig2. Detection of human CB1 expression in Brains, liver and kidney in hCB1 knockin mice by WB.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig3. Detection of human CB1 expression in CA1 in hCB1 knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig4. Detection of human CB1 expression in CA3 in hCB1 knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig5. Detection of human CB1 expression in dentate gyrus(DG) in hCB1 knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig6. Detection of human CB1 expression in cerebellum in hCB1 knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
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