CAG-LSL-dCas9-SPH-Tg

Nomenclature

C57BL/6Smoc-Tg(CAG-LSL-dCas9-SPH)Smoc

Cat. NO.

NM-TG-00025

Strain State

Repository Live

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Gene Summary

Gene Symbol

Synonyms

Null

NCBI ID

Null

MGI ID

Null

Ensembl ID

Null

Pubmed

Model Description

Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher).
Research Application：gene regulation tool,CRISPRa

Publications

In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9- activator transgenic mice
References：Nature Neuroscience

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