hMERTK

Nomenclature

C57BL/6Smoc-Mertkem1(hMERTK)/Smoc

Cat. NO.

NM-HU-2000031

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
Mertk

Model Description

The endogenous mouse Mertk gene was replaced by human MERTK gene.
Research Application:Immunotherapy,drug screening

Validation Data

image.png

Fig1. Detection of MERTK expression by RT-PCR. 

Wild type: only one band at 201 bp with primers F2/R2(mMertk);

Homozygous: only one band at 122 bp with primers F1/R1(hMERTK); 

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

hMERTK-1.png

Fig2. Human MERTK expression on lymph nodes is confirmed by Western Blot in the humanized MERTK mice. 

Human MERTK expresses on lymph nodes in the humanized MERTK mice.image.png

Fig3. Detection of hMerTK expression on human PBMC.

image.png

Fig4. Detection of hMerTK expression on BMDM and BMDC in hMerTK KI mice. 

Abbr. BMDM, Bone Marrow Derived Macrophage; BMDC, Bone Marrow Derived Dendritic cell.

hMERTK-2.png

Fig5. Human MERTK expression on spleen cells is confirmed by immunofluorescence in the humanized MERTK mice. 

Human MERTK expresses obviously on spleen cells in the humanized MERTK mice.

hMERTK-3.png

Fig6. Human MERTK expression on thymus is confirmed by immunofluorescence in the humanized MERTK mice. 

Human MERTK expresses obviously on thymus in the humanized MERTK mice.


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