Wt1-CreERT2

Nomenclature

C57BL/6Smoc-Wt1em1(CreERT2-WPRE-pA)Smoc

Cat. NO.

NM-KI-200127

Strain State

Repository Live

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Gene Summary

Gene Symbol
Wt1

Synonyms

Wt-1; D630046I19Rik

NCBI ID

22431

MGI ID

98968

Ensembl ID

ENSMUSG00000016458

Pubmed

Wt1

Human Ortholog

WT1

Model Description

A CreERT2-WPRE-pA expression cassette was knocked into the Wt1 gene start codon site.
Research Application：As Wt1 is expressed in the epicardium of the heart and in the mesothelia overlying most visceral organs, these mutant mice are useful for lineage-tracing/marking Wt1-expressing cells for studying the heart, the development of sperm and hepatic injury.

Validation Data

NM-KI-200127 Wt1-CreERT2-1.png

Fig. 1 CreERT2-mediated recombination in the heart of Wt1CreERT2/+; Rosa26tdTomato/+ mouse. 

Detection of tdTomato (red) in the epicardium of Wt1CreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

NM-KI-200127 Wt1-CreERT2-2.png

Fig. 2 CreERT2-mediated recombination in the heart of Wt1CreERT2/+; Rosa26tdTomato/+ mouse embryo at E10.5. 

Detection of tdTomato (red) in the heart of Wt1CreERT2/+; Rosa26tdTomato/+ mouse embryo at E10.5 after tamoxifen treatment.

NM-KI-200127 Wt1-CreERT2-3.png

Fig. 3 CreERT2-mediated recombination in the liver of Wt1CreERT2/+; Rosa26tdTomato/+ mouse. 

Detection of tdTomato (red) in the mesothelial cell of the liver derived from Wt1CreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

NM-KI-200127 Wt1-CreERT2-4.png

Fig. 4 CreERT2-mediated recombination in the testis of Wt1CreERT2/+; Rosa26tdTomato/+ mouse. 

Detection of tdTomato (red) in the tunica albuginea, tunica vaginalis and individual cells of parenchymal structure of the testis derived from Wt1CreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

NM-KI-200127 Wt1-CreERT2-5.png

Fig. 5: Detection of tdTomato(red) in various tissues of Wt1CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can be detected in the tunica externa of heart, liver, spleen, stomach, intestine, colon after tamoxifen treatment. Some leakiness were detected in the spleen prior to tamoxifen exposure. Besides, tdtomato expression can be detected in a few cells of the pancreas and thymus. Tdtomato expression can not be observed in the brain, pituitary gland, retina, lung, submandibular gland, skin and muscle. (For more detailed information please contact our technical advisor.)

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