Zap70-W163C(Balb/c)

Nomenclature

BALB/cAnSmoc-Zap70em(W163C)Smoc

Cat. NO.

NM-KI-210366

Strain State

Repository Live

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Gene Summary

Gene Symbol
Zap70

Synonyms

Srk; mur; mrtle; ZAP-70

NCBI ID

22637

MGI ID

99613

Ensembl ID

ENSMUSG00000026117

Pubmed

Zap70

Human Ortholog

ZAP70

Model Description

These mice carry a W163C mutation of Zap70 gene.
Research Application：Rheumatoid arthritis model， Ankylosing spondylit，SKG mouse arthritis modelis model

Disease Connection

Rheumatoid Arthritis

Phenotype(s)

MGI:3698734

Reference(s)

Sakaguchi N, Takahashi T, Hata H, Nomura T, Tagami T, Yamazaki S, Sakihama T, Matsutani T, Negishi I, Nakatsuru S, Sakaguchi S, Altered thymic T-cell selection due to a mutation of the ZAP-70 gene causes autoimmune arthritis in mice. Nature. 2003 Nov 27;426(6965):454-60

Validation Data

image.png

Fig.1 Clinical score of joint swelling in HO Zap70-W163C (Balb/C) mice(A) and representative diagram of hind limb joints of mice in each group on day 52(B) (female, 6-8wks). Compared with the control group, the mannan-induced mice gradually increased their joint swelling scores over time.

Signs of arthritis were assessed by a visual score where 0= Normal paw, 1 = one toe inflamed or swollen, 2 = more than one toe, but not entire paw, or mild swelling of the entire paw, 3 = entire paw inflamed and swollen, and 4 = very inflamed and swollen paw or ankylosed paw.

image.png

Fig.2 Hindlimb micro-CT image of HO Zap70-W163C(Balb/C) mice in  the control (G1) and model groups (G2) (female, 6-8wks). The orange arrows point to osteophytes.

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Fig.3 Score of HO Zap70-W163C(Balb/C) mice (A) and H&E pathology(B). (female, 6-8wks, n=3)

Compared with G1, the infiltration of mixed inflammatory cells in the induced group was significantly increased, and the synovial membrane of the joints showed obvious hyperplasia, pannu formation, fibrous tissue formation, and cartilage erosion.

Publications

A novel Anti-ROS osteoblast-specific delivery system for ankylosing spondylitis treatment via suppression of both inflammation and pathological new bone formation
References：Journal of Nanobiotechnology

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