hTHPO(NMG)

The hTHPO(NM-HU-2000062) mice were backcrossed to NMG genetic background, to create the new strain of hTHPO(NMG) (NM-HU-220429) mice.

Fig.1 Detection of THPO expression in adrenal and liver by RT-PCR. 

Wild type: only one band at 166 bp with primers F1/R1(mThpo);

Homozygous: only one band at 167 bp with primers F2/R2(hTHPO).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of hTHPO expression in serum and liver homogenate by ELISA (male, 6-11wks).

Abbr. HO, Homozygous; WT, wild type.

Note. hTHPO(NMG) and NMG mice were i.p. injected with LPS. The Human Thrombopoietin ELISA Kit cross-reacted with mouse THPO and humanized THPO.

Fig.3 Detection of hTHPO expression in hTHPO(NMG) mice by WB. 

Abbr. M, marker; WT, wild type; PC, positive control, Hela cells.

Note. The anti-human THPO Antibody cross-reacted with mouse THPO and humanized THPO.